报告题目：Cryo-EM analysis of spliceosome: The assembly of spliceosome and mechanism of pre-mRNA splicing

报告人： Chuangye Yan  闫创业

               Life school of Tsinghua University

摘要: Pre-mRNA splicing is carried out by the spliceosome, which undergoes an intricate assembly and activation process. By applying single-particle cryo-EM, we solved several high resolution spliceosomal complexes at different stage of pre-mRNA splicing, including intron-lariat spliceosome, U4/U6.U5 tri-snRNP, B^act complex, and C complex. These near-atomic structures provide detailed information for spliceosome assembly and mechanism of pre-mRNA splicing.

个人简介：

Education:

2015-present, Postdoctoral fellow in Life School of Tsinghua University, Advisor: Prof. Yigong Shi.

2009 - 2015，Ph.D. candidate in Life School of Tsinghua University, Advisor: Prof. Yigong Shi

2005 -2009，Undergraduate education in the Department of Chemistry, Tsinghua University

Research interesting:

Current research is focused on single particle Cryo-EM analysis of spliceosome and mechanism of pre-mRNA splicing.

Representative Publication:

1         Yan, C.*, Wan, R., Bai, R., Huang, G., Shi, Y. Structure of a yeast catalytically activated spliceosome at 3.5 A resolution. Science,  (2016); published online EpubJul 21 (10.1126/science.aag0291).

2         Wan, R., Yan, C.*, Bai, R,. Huang, G., Shi, Y. Structure of a yeast catalytic step I spliceosome at 3.4 A resolution. Science,  (2016); published online EpubJul 21 (10.1126/science.aag2235).

3         Wan, R., Yan, C.*, Bai, R., Wang, L., Huang, M., Wong, C.C., and Shi, Y. The 3.8 A structure of the U4/U6.U5 tri-snRNP: Insights into spliceosome assembly and catalysis. Science 351, 466-475, doi:10.1126/science.aad6466 (2016).

4         Yan, C.*, Hang, J., Wan, R., Huang, M., Wong, C.C., and Shi, Y. Structure of a yeast spliceosome at 3.6-angstrom resolution. Science 349, 1182-1191, doi:10.1126/science.aac7629 (2015).

5         Hang, J., Wan, R., Yan, C.* & Shi, Y. Structural basis of pre-mRNA splicing. Science 349, 1191-1198, doi:10.1126/science.aac8159 (2015).

6        Bai, X. C., Yan, C.*, Yang, G., Lu, P., Ma, D., Sun, L., Zhou, R., Scheres, S.H., and Shi, Y. An atomic structure of human gamma-secretase. Nature 525, 212-217, doi:10.1038/nature14892 (2015)..

7        Deng, D., Yan, C.*, Pan, X., Mahfouz, M., Wang, J., Zhu, J.K., Shi, Y., and Yan, N. Structural basis for sequence-specific recognition of DNA by TAL effectors. Science 335, 720-723, doi:10.1126/science.1215670 (2012).

报告时间： 2016年8月19日 9:00-9:40

报告地点：人民大学环境楼316会议室